Sound coming out of one speaker only

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Hi, I am new to this board and this is my first post. I have pretty good computer knowledge and assembled by computer about 3 years ago.

I have an ASUS A7N8X Deluxe motherboard. My problem is sound only comes out of one speaker in a 2 speaker set up. Nothing fancy just a 2 speaker set up. Used to get sound out of both speakers and then had to store computer to make room for some painting. When reattached speakers to computer, sound only comes from one speaker. I tried another set of speakers with the same result.

I have downloaded latest NVidia audio drivers but that didn't help. Anybody have any suggestions.
 
Have tried a second set of speakers. Same result. Turned computer off, plugged original speakers into front audio jack and booted up. Both speakers now work, but the Microsoft master volume control panel does not work though individual volume control for wav, etc. does.

Could master volume control file be corrupt?
 
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