We moved my computer upstairs, and now my speakers won't work. I checked all plugs 10 times, and think it's right. I have windows Vista. Please help.

push front speaker out

Make sure your volume is turned up in windows.
Your speakers should be plugged in to the light green connector on the back of your pc.
Try the speakers in a diffrent system, an ipod or walkman will do
Try a set of headphones in the headphone jack on your pc.

This will help narrow down the problem.

Maybe an obvious question, but sometimes...

Are the speakers attached to power and "power on"?

Best to first test if your speakers are working, it's possible one of the wires has broken inside when you took the system apart to move it.
Test it by; Pull the speaker plug out of the back of PC, plug it into an MP3 player or radio.
Does it amplify the sound ? If not, is the volume turned up enough on the speaker? Is there a light on one speaker to show they are on? Possible blown fuse?
If it does, plug back into the PC Green and Blue sockets, (not Pink).
Click on the speaker icon, near the time, in your taskbar, move the marker up to about 1/3. It should work.