The motherboard spec says PCI-x 16.
Will a PCI-X16 2.0 work?
Without know your Hardware manufacture and model, it's difficult to say
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It might but the proformance of the card will be limited to what it could really do.
No it won't, suggest you hear the other members opinions
2.0 is backwards compatible, you'll be fine.
Does the MoBo hold the tech or the graphics card
Both "hold the tech". A PCI-E 2.0 interconnect requires both a PCI-E 2.0 video card and a PCI-E 2.0 slot on the motherboard. That said, on most motherboards, PCI-E 2.0 is backwards compatible, so a PCI-E 2.0 card will work on a PCI-E slot and a PCI-E card will work in a PCI-E 2.0 slot.
Performance differences are next to non-existant on all but the most powerful video cards, to boot.
i second this, im running a gtx280 in a pci-e x16 (not 2.0) and it works just like it would in a pci-e x16 2.0 slot.